TY - JOUR
T1 - The use of NaCl addition for the improvement of polyhydroxyalkanoate production by Cupriavidus necator
AU - Passanha, Pearl
AU - Kedia, Gopal
AU - Dinsdale, Richard M.
AU - Guwy, Alan J.
AU - Esteves, Sandra R.
PY - 2014/7/1
Y1 - 2014/7/1
N2 - External stress factors in the form of ionic species or temperature increases have been shown to produce a stress response leading to enhanced PHA production. The effect of five different NaCl concentrations, namely 3.5, 6.5, 9, 12 and 15 g/l NaCl on PHA productivity using Cupriavidus necator has been investigated alongside a control (no added NaCl). A dielectric spectroscopy probe was used to measure PHA accumulation online in conjunction with the chemical offline analysis of PHA. The highest PHA production was obtained with the addition of 9 g/l NaCl, which yielded 30% higher PHA than the control. Increasing the addition of NaCl to 15 g/l was found to inhibit the production of PHA. NaCl addition can therefore be used as a simple, low cost, sustainable, non toxic and non reactive external stress strategy for increasing PHA productivity.
AB - External stress factors in the form of ionic species or temperature increases have been shown to produce a stress response leading to enhanced PHA production. The effect of five different NaCl concentrations, namely 3.5, 6.5, 9, 12 and 15 g/l NaCl on PHA productivity using Cupriavidus necator has been investigated alongside a control (no added NaCl). A dielectric spectroscopy probe was used to measure PHA accumulation online in conjunction with the chemical offline analysis of PHA. The highest PHA production was obtained with the addition of 9 g/l NaCl, which yielded 30% higher PHA than the control. Increasing the addition of NaCl to 15 g/l was found to inhibit the production of PHA. NaCl addition can therefore be used as a simple, low cost, sustainable, non toxic and non reactive external stress strategy for increasing PHA productivity.
KW - Polyhydroxyalkanoate production
KW - Cupriavidus necator
KW - Sodium chloride
KW - Volatile fatty acids
KW - Dielectric spectroscopy
U2 - 10.1016/j.biortech.2014.04.068
DO - 10.1016/j.biortech.2014.04.068
M3 - Article
SN - 0960-8524
VL - 163
SP - 287
EP - 294
JO - Bioresource Technology
JF - Bioresource Technology
ER -