Responses of mouse spinal neurones in culture to locally applied Phe-Met-Arg-Phe-NH2

P. W. McCarthy; G. A. Cottrell

doi:10.1016/0742-8413(84)90218-4

Responses of mouse spinal neurones in culture to locally applied Phe-Met-Arg-Phe-NH2

P. W. McCarthy^*, G. A. Cottrell

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

19 Citations (Scopus)

Abstract

1. Embryonic mouse spinal cord neurones were maintained in a primary dissociated culture with a medium free from antibiotics.

2. Intracellular recordings were made and the effects of local application of the neuropeptide FMRFamide were tested on selected neurones. Two types of response were seen.

3. One response was depolarizing, with an accompanying decrease in conductance. This response was probably caused by a reduction in permeability to potassium ions.

4. The second type of response was accompanied by an increase in conductance. Such responses showed a wide variation in their reversal potentials between different neurones. A combination of permeability changes to sodium and chloride ions appeared to be responsible for these responses.

Original language	English
Pages (from-to)	383-387
Number of pages	5
Journal	Comparative Biochemistry and Physiology. Part C, Comparative Pharmacology
Volume	79
Issue number	2
DOIs	https://doi.org/10.1016/0742-8413(84)90218-4
Publication status	Published - 1984
Externally published	Yes

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title = "Responses of mouse spinal neurones in culture to locally applied Phe-Met-Arg-Phe-NH2",

abstract = "1. Embryonic mouse spinal cord neurones were maintained in a primary dissociated culture with a medium free from antibiotics. 2. Intracellular recordings were made and the effects of local application of the neuropeptide FMRFamide were tested on selected neurones. Two types of response were seen. 3. One response was depolarizing, with an accompanying decrease in conductance. This response was probably caused by a reduction in permeability to potassium ions. 4. The second type of response was accompanied by an increase in conductance. Such responses showed a wide variation in their reversal potentials between different neurones. A combination of permeability changes to sodium and chloride ions appeared to be responsible for these responses. ",

author = "McCarthy, {P. W.} and Cottrell, {G. A.}",

year = "1984",

doi = "10.1016/0742-8413(84)90218-4",

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AU - McCarthy, P. W.

AU - Cottrell, G. A.

PY - 1984

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N2 - 1. Embryonic mouse spinal cord neurones were maintained in a primary dissociated culture with a medium free from antibiotics. 2. Intracellular recordings were made and the effects of local application of the neuropeptide FMRFamide were tested on selected neurones. Two types of response were seen. 3. One response was depolarizing, with an accompanying decrease in conductance. This response was probably caused by a reduction in permeability to potassium ions. 4. The second type of response was accompanied by an increase in conductance. Such responses showed a wide variation in their reversal potentials between different neurones. A combination of permeability changes to sodium and chloride ions appeared to be responsible for these responses.

AB - 1. Embryonic mouse spinal cord neurones were maintained in a primary dissociated culture with a medium free from antibiotics. 2. Intracellular recordings were made and the effects of local application of the neuropeptide FMRFamide were tested on selected neurones. Two types of response were seen. 3. One response was depolarizing, with an accompanying decrease in conductance. This response was probably caused by a reduction in permeability to potassium ions. 4. The second type of response was accompanied by an increase in conductance. Such responses showed a wide variation in their reversal potentials between different neurones. A combination of permeability changes to sodium and chloride ions appeared to be responsible for these responses.

U2 - 10.1016/0742-8413(84)90218-4

DO - 10.1016/0742-8413(84)90218-4

M3 - Article

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JO - Comparative Biochemistry and Physiology. Part C, Comparative Pharmacology

JF - Comparative Biochemistry and Physiology. Part C, Comparative Pharmacology

IS - 2

ER -

Responses of mouse spinal neurones in culture to locally applied Phe-Met-Arg-Phe-NH2

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