Reclassification of the specialized metabolite producer Pseudomonas mesoacidophila ATCC 31433 as a member of the Burkholderia cepacia complex

E. Joel Loveridge*, Cerith Jones, Matthew J. Bull, Suzy C. Moody, Malgorzata W. Kahl, Zainab Khan, Louis Neilson, Marina Tomeva, Sarah E. Adams, Andrew C. Wood, Daniel Rodriguez-Martin, Ingrid Pinel, Julian Parkhill, Eshwar Mahenthiralingam, John Crosby

*Corresponding author for this work

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    3 Citations (Scopus)
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    Abstract

    Pseudomonas mesoacidophila ATCC 31433 is a Gram-negative bacterium, first isolated from Japanese soil samples, that produces the monobactam isosulfazecin and the β-lactam-potentiating bulgecins. To characterize the biosynthetic potential of P. mesoacidophila ATCC 31433, its complete genome was determined using single-molecule real-time DNA sequence analysis. The 7.8-Mb genome comprised four replicons, three chromosomal (each encoding rRNA) and one plasmid. Phylogenetic analysis demonstrated that P. mesoacidophila ATCC 31433 was misclassified at the time of its deposition and is a member of the Burkholderia cepacia complex, most closely related to Burkholderia ubonensis. The sequenced genome shows considerable additional biosynthetic potential; known gene clusters for malleilactone, ornibactin, isosulfazecin, alkylhydroxyquinoline, and pyrrolnitrin biosynthesis and several uncharacterized biosynthetic gene clusters for polyketides, nonribosomal peptides, and other metabolites were identified. Furthermore, P. mesoacidophila ATCC 31433 harbors many genes associated with environmental resilience and antibiotic resistance and was resistant to a range of antibiotics and metal ions. In summary, this bioactive strain should be designated B. cepacia complex strain ATCC 31433, pending further detailed taxonomic characterization.

    Original languageEnglish
    Article numbere00125-17
    Number of pages12
    JournalJournal of Bacteriology
    Volume199
    Issue number13
    DOIs
    Publication statusPublished - 1 Jul 2017

    Keywords

    • Antibacterial
    • Antibiotic resistance
    • Biosynthesis
    • Bulgecin
    • Genome
    • Identification
    • Metal resistance

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