Production of Poly-γ-glutamic acid by Bacillus subtilis and Bacillus licheniformis with different growth media

Gopal Kedia, D. Hill, R. Hill, I. Radecka

Research output: Contribution to journalArticlepeer-review

Abstract

Poly ?-glutamic acid is a naturally occurring homo-polyamide that is made of D- and L-glutamic acid units connected by amide linkages between a-amino and ?-carboxylic groups. Many strains have been studied for the production of poly ?-glutamic acid, B. licheniformis 9945a and B. subtilis natto are the two most widely studied strains due to their higher yield. Both of these strains require L-glutamic acid for poly ?-glutamic acid production. This paper describes investigations on the biosynthesis of poly ?-glutamic acid by B. licheniformis 9945a and B. subtilis natto in an automated pH-controlled fermenter. The effect of the growth media such as medium E, F, C and GS was investigated. Medium E produced poly ?-glutamic acid both with and without MnSO4 in the medium when fermented by B. licheniformis. Presence of MnSO4 in the medium affected both the yield and biomass production. The yield was 12 g/l for the medium containing 2.46 mM MnSO4 whereas it was only 4-5 g/l for the medium without MnSO4 with molecular weight (Mw) of 321,000. Different media such as medium C, F and GS were used for production of poly ?-glutamic acid by B. subtilis natto. The yield from GS medium was highest and it produced about 26-28 g/l with molecular weight (Mw) of 627,000.
Original languageEnglish
Pages (from-to)5926 - 5934
Number of pages8
JournalJournal of Nanoscience and Nanotechnology
Volume10
Issue number9
DOIs
Publication statusPublished - 9 Sep 2010

Keywords

  • poly-γ-glutamic acid
  • bacillus licheniformis
  • bacillus subtilis

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