TY - JOUR
T1 - Production and characterization of medium-chain-length polyhydroxyalkanoate copolymer from Arctic psychrotrophic bacterium Pseudomonas sp. PAMC 28620
AU - Sathiyanarayanan, Ganesan
AU - Bhatia, Shashi Kant
AU - Song, Hun-Suk
AU - Jeon, Jong-Min
AU - Kim, Junyoung
AU - Lee, Yoo Kyung
AU - Kim, Yun-Gon
AU - Yang, Yung-Hun
N1 - Funding Information:
The author G. Sathiyanarayanan would like to thank KU Brain Pool Fellowship Program of Konkuk University, Seoul, South Korea. This study was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2016R1D1A1B03932301) and (NRF-2015M1A5A1037196), and Korea Polar Research Institute (PE16030). Consulting service from the Microbial Carbohydrate Resource Bank (MCRB, Seoul, South Korea) was kindly appreciated.
Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2017/4/1
Y1 - 2017/4/1
N2 - Arctic psychrotrophic bacterium Pseudomonas sp. PAMC 28620 was found to produce a distinctive medium-chain-length polyhydroxyalkanoate (MCL-PHA) copolymer when grown on structurally unrelated carbon sources including glycerol. The maximum MCL-PHA copolymer yield was obtained about 52.18 ± 4.12% from 7.95 ± 0.66 g/L of biomass at 144 h of fermentation when 3% glycerol was used as sole carbon and energy source during the laboratory-scale bioreactor process. Characterization of the copolymer was carried out using fourier transform infrared spectroscopy (FTIR), gas chromatography–mass spectrometry (GC–MS), proton (1H) and carbon (13C) nuclear magnetic resonance spectroscopy (NMR), gel permeation chromatography (GPC), differential scanning calorimeter (DSC) and thermo-gravimetric analysis (TGA). The copolymer produced by Pseudomonas sp. PAMC 28620 consisting of four PHA monomers and identified as 3-hydroxyoctanoate (3HO), 3-hydroxydecanoate (3HD), 3-hydroxydodecanoate (3HDD) and 3-hydroxytetradecanoate (3HTD). An average molecular weight of the copolymer was found approximately 30.244 kDa with polydispersity index (PDI) value of 2.05. Thermal analysis showed the produced MCL-PHA copolymer to be low-crystalline (43.73%) polymer with great thermal stability, having the thermal decomposition temperature of 230 °C–280 °C, endothermic melting temperature (Tm) of 172.84 °C, glass transition (Tg) temperature of 3.99 °C, and apparent melting enthalpy fusion (ΔHm) about 63.85 J g−1.
AB - Arctic psychrotrophic bacterium Pseudomonas sp. PAMC 28620 was found to produce a distinctive medium-chain-length polyhydroxyalkanoate (MCL-PHA) copolymer when grown on structurally unrelated carbon sources including glycerol. The maximum MCL-PHA copolymer yield was obtained about 52.18 ± 4.12% from 7.95 ± 0.66 g/L of biomass at 144 h of fermentation when 3% glycerol was used as sole carbon and energy source during the laboratory-scale bioreactor process. Characterization of the copolymer was carried out using fourier transform infrared spectroscopy (FTIR), gas chromatography–mass spectrometry (GC–MS), proton (1H) and carbon (13C) nuclear magnetic resonance spectroscopy (NMR), gel permeation chromatography (GPC), differential scanning calorimeter (DSC) and thermo-gravimetric analysis (TGA). The copolymer produced by Pseudomonas sp. PAMC 28620 consisting of four PHA monomers and identified as 3-hydroxyoctanoate (3HO), 3-hydroxydecanoate (3HD), 3-hydroxydodecanoate (3HDD) and 3-hydroxytetradecanoate (3HTD). An average molecular weight of the copolymer was found approximately 30.244 kDa with polydispersity index (PDI) value of 2.05. Thermal analysis showed the produced MCL-PHA copolymer to be low-crystalline (43.73%) polymer with great thermal stability, having the thermal decomposition temperature of 230 °C–280 °C, endothermic melting temperature (Tm) of 172.84 °C, glass transition (Tg) temperature of 3.99 °C, and apparent melting enthalpy fusion (ΔHm) about 63.85 J g−1.
KW - Arctic Pseudomonas
KW - Copolymer
KW - Fermentation
KW - Glycerol
KW - Polyhydroxyalkanoates
U2 - 10.1016/j.ijbiomac.2017.01.053
DO - 10.1016/j.ijbiomac.2017.01.053
M3 - Article
C2 - 28108411
AN - SCOPUS:85010382757
SN - 0141-8130
VL - 97
SP - 710
EP - 720
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -