Performance of a novel molecular test designed for Point-of-Care UTI diagnosis

Ayako Van Der Goes Van Naters, Joanna Diggle, Shanine Mitchell, Ali Roula, Jeroen Nieuwland, Emma Hayhurst

Research output: Contribution to conferencePosterpeer-review

Abstract

The current ‘gold standard’ method for UTI diagnosis is the culture method. However, it is time consuming (24-72 hours), requires expensive equipment, a high volume of consumables and highly skilled laboratory staff to interpret and report results.

Clinical decisions are often made on the basis of a urine dipstick test and patient symptoms at the point of care. The inaccuracies of these methods and the slow return of culture results means infections are often missed, or antibiotics are given out inappropriately or unnecessarily. This increases the risk of resistant, chronic/recurrent or severe infections developing, with negative impacts on patient outcomes.

149 randomly selected fresh urines were tested using Lodestar DX (LAMP) technology, alongside laboratory culture method at UHW, Cardiff Microbiology.

Urine samples (1 μL) were added directly to the E.coli assay

Results were shown and recorded after 40 minutes as ‘positive’ or ‘negative’ according to the decision of Lodestar’s algorithm

Lodestar DX results were compared to standard culture results and sensitivity and specificity calculated.

Sensitivity and specificity for E.coli was high and the test system may be better at diagnosing mixed infections which represent a high proportion of all culture results.
The test system is easy to use with no sample processing and minimal training requirements. Test results are available in 40 minutes, compared to 24-72 hours for culture results.
The performance of the test system for the detection of other uropathogens (Klebsiella, Enterococcus, Pseudomonas, Proteus and S. saprophyticus) is ongoing. Sensitivity can be further improved by refining the test method.

Original languageEnglish
Pages1
Number of pages1
Publication statusPublished - Jun 2023
EventThe British Society for Antimicrobial Chemotherapy, UTI conference: The Challenge of Urinary Tract Infections - Royal College of Physicians London, London
Duration: 14 Jun 2023 → …
https://bsac.org.uk/uti-conference-2023/

Conference

ConferenceThe British Society for Antimicrobial Chemotherapy, UTI conference
Abbreviated titleBSAC, UTI conference 2023
CityLondon
Period14/06/23 → …
Internet address

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