Off-line two-dimensional LC-tandem MS of menaquinones from thermophilic bacteria

Elizaveta Timkina, Irena Jarošová Kolouchová, Lucie Kyselová, Andrea Palyzová, Denis J. Murphy, Tomáš Řezanka*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review


Thermophilic bacteria of four genera in contrast to the commonly used production strains such as Bacillus subtilis, produce homologs other than menaquinone (MK) with seven isoprene units. The number of isoprene units and the configuration of double bonds are essential factors for their biological activity. The goal was to obtain a strain of bacteria that produces a wide range of MK homologs and only all-trans geometrical isomers, which was the strain G. kaustophilus. Using off-line two-dimensional LC-tandem MS in columns with the RP18 phase and the COSMOSIL cholester phase (separation according to the geometric configuration of double bonds) it was shown that thermophilic bacteria grown at different temperatures produce only all-trans isomers of menaquinones from MK-5 (menaquinone with five isoprenyl units) to MK-15 (fifteen isoprenyl units). Therefore, G. kaustophilus appears to be a biotechnologically important strain produces only trans isomers and additionally homologs from 5 to 15 isoprene units.

Original languageEnglish
Article number137112
Number of pages8
JournalFood Chemistry
Early online date6 Aug 2023
Publication statusPublished - 11 Aug 2023


  • Thermophilic bacteria
  • Menaquinones
  • Shotgun mass spectrometry
  • Cis/trans isomers
  • LC/ESI–tandem MS


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