Abstract
A Serratia sp. expresses a high level of acid phosphatase when grown continuously under carbon limitation. In the presence of CaCl2, biosynthesis of nanocrystalline hydroxyapatite (bio-HA) was achieved by utilizing phosphate released via enzymatic cleavage of an applied substrate (glycerol 2-phosphate: G2P). Hydroxyapatite crystals were identified by energy dispersive X-ray emission (EDX) and selected area diffraction (SAD). X-ray powder diffraction (XRD) analysis gave a mean crystallite size of »21–32 nm, with the smallest crystals (21–24 nm) obtained using 1 mM Ca2C and 1 mM G2P. The uptake of Eu3C and Sr2C by bio-HA made by continuously pregrown cells (0.42 mg/mg and 0.043 mg/mg respectively) was »20% greater for Sr2C than was previously reported for bio-HA material of size »40 nm made by batchpregrown cells, while the corresponding uptake of Eu3C was increased by > 1.8-fold. This was attributed to the localization of Eu (III) at grain boundaries by reference to previous work and highlights th...
| Original language | English |
|---|---|
| Pages (from-to) | 267-273 |
| Number of pages | 7 |
| Journal | Geomicrobiology Journal |
| Volume | 33 |
| Issue number | 3-4 |
| DOIs | |
| Publication status | E-pub ahead of print - 14 Aug 2015 |