Abstract
Biogenic hydroxyapatite (bio-HA) has the potential for radionuclide capture and remediation of metal contaminated environments. Biosynthesis of bio-HA was achieved via the phosphatase activity of a Serratia sp. supplemented with various concentrations of CaCl2 and glycerol 2-phosphate (G2P) provided at pH 7.0 or 8.6. Presence of hydroxyapatite (HA) was confirmed in the samples by X-ray powder diffraction analysis. When provided with limiting (1 mM) G2P and excess (5 mM) Ca2C at pH 8.6, monohydrocalcite was found. This, and bio-HA with less (1 mM) Ca2C accumulated Eu(III) to »31% and 20% of the biomineral mass, respectively, as compared to 50% of the mineral mass accumulated by commercial HA. Optimally, with bio-HA made at initial pH 7.0 from 2 mM Ca2C and 5 mM G2P, Eu(III) accumulated to »74% of the weight of bio-HA, which was equal to the mass of the HA mineral component of the biomaterial. The implications with respect to potential bio-HA-barrier development in situ or as a remediation strategy are discussed.
| Original language | English |
|---|---|
| Pages (from-to) | 753-759 |
| Number of pages | 7 |
| Journal | Geomicrobiology Journal |
| Volume | 34 |
| Issue number | 9 |
| Early online date | 23 Feb 2017 |
| DOIs | |
| Publication status | E-pub ahead of print - 23 Feb 2017 |