Electron paramagnetic spectroscopic evidence of exercise-induced free radical accumulation in human skeletal muscle

Damian Bailey, Leslie Lawrenson, Jane McEneny, Ian S. Young, Philip E. James, Simon K. Jackson, Robert R. Henry, Odile Mathieu-Costello, Joe M. McCord, Russell S. Richardson

Research output: Contribution to journalArticlepeer-review

Abstract

The present study determined if acute exercise increased free radical formation in human skeletal muscle. Vastus lateralis biopsies were obtained in a randomized balanced order from six males at rest and following single-leg knee extensor exercise performed for 2 min at 50% of maximal work rate (WRMAX) and 3 min at 100% WRMAX. EPR spectroscopy revealed an exercise-induced increase in mitochondrial ubisemiquinone [0.167 ± 0.055 vs. rest: 0.106 ± 0.047 arbitrary units (AU)/g total protein (TP), P andlt; 0.05] and a-phenyl-tert-butylnitrone-adducts (112 ± 41 vs. rest: 29 ± 9 AU/mg tissue mass, P andlt; 0.05). Intramuscular lipid hydroperoxides also increased (0.320 ± 0.263 vs. rest: 0.148 ± 0.071 nmol/mg TP, P andlt; 0.05) despite an uptake of a-tocopherol, a-carotene and ß-carotene. There were no relationships between mitochondrial volume density and any biomarkers of oxidative stress. These findings provide the first direct evidence for intramuscular free radical accumulation and lipid peroxidation following acute exercise in humans.
Original languageEnglish
Pages (from-to)182 - 190
Number of pages8
JournalFree Radical Research
Volume42
Issue number2
DOIs
Publication statusPublished - 1 Feb 2007

Keywords

  • epr
  • spin-trapping
  • lipid peroxidation
  • antioxidants
  • skeletal muscle
  • mitochondria

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