Effect of acetate concentration, temperature, pH and nutrient concentration on polyhydroxyalkanoates (PHA) production by glycogen accumulating organisms

Marie-Claire Catherine*, Alan Guwy, Jaime Massanet-Nicolau

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Low PHA accumulation is one of the main limitations of the industrial production of polyhydroxyalkanoates (PHA) from mixed cultures. Different PHA yields and physical properties were obtained by varying culture conditions (acetate concentration, nitrogen and phosphate availability, temperature and pH). Glycogen accumulating organisms (GAOs), microorganisms able to create and store PHA, which use acetate as their sole carbon source, were obtained after selection from mixed culture from activated sludge. All PHA presented good adhesion properties and were soluble in halogenated and polar solvents. The maximum PHA storage rate (20 % of cell dry weight) was achieved with a concentration of 4 g/L acetate. The optimum temperature for PHA production was 23 °C (24 % of cell dry weight). Low nitrogen concentration increased the yield (22 %) while phosphate concentration and pH did not influence PHA production. PHA accumulation of almost 30 % was obtained with previously found optimum parameters.

Original languageEnglish
Article number101226
Number of pages8
JournalBioresource Technology Reports
Volume20
Early online date23 Sep 2022
DOIs
Publication statusE-pub ahead of print - 23 Sep 2022

Keywords

  • Acetate
  • GAO
  • Mixed culture
  • PHA

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