Abstract
The binding of tetracationic - and -europium complexes of tetraamide and triamide ligands incorporating an N-methylphenanthridinium group to calf-thymus DNA, poly(dGdC) and super-coiled plasmid DNA has been monitored by absorption, luminescence and CD difference spectroscopy. With the tetraamide complexes, preferential binding to (CG) base-pairs was signalled by pronounced hypochromism, a small red shift and substantial fluorescence quenching with a limiting base-pair complex ratio of 2 : 1, consistent with an intercalative interaction and charge neutralisation. For the triamide Eu complex, a lower base-pair : complex ratio was found and changes in the form and polarisation of the Eu emission spectrum were consistent with coordination of a nucleobase phosphate group to the hydrated Eu centre.
Original language | English |
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Pages (from-to) | 1738-1741 |
Number of pages | 4 |
Journal | Journal of the Chemical Society, Perkin Transactions 2 |
Issue number | 9 |
DOIs | |
Publication status | Published - 1 Oct 2001 |
Externally published | Yes |