Coherent Raman Scattering Microscopy: Technology Developments and Biological Applications

I. Pope; F. Masia; J. Bradley; K. Ewan; A. Karuna; L. Payne; I. Guschina; J. Harwood; K. Swann; T. Dale; P. Watson; W. Langbein; P. Borri

doi:10.1109/ICTON.2018.8473706

Coherent Raman Scattering Microscopy: Technology Developments and Biological Applications

I. Pope, F. Masia, J. Bradley, K. Ewan, A. Karuna, L. Payne, I. Guschina, J. Harwood, K. Swann, T. Dale, P. Watson, W. Langbein, P. Borri

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Optical microscopy is an indispensable tool that is driving progress in cell biology, and is still the only practical means of obtaining spatial and temporal resolution within living cells and tissues. Coherent Raman Scattering (CRS) microscopy has emerged in the last decade as a powerful multiphoton microscopy technique which overcomes the need for fluorescent labelling and yet retains biomolecular specificity and intrinsic 3D resolution. We have developed several home-built CRS microscopes featuring innovative excitation/detection schemes and quantitative image analysis. Our second-generation multimodal CRS instrument is based on a single 5fs Ti:Sa laser source with 350nm bandwidth capable of exciting a wide vibrational range from 1200 cm^-1 to 3800 cm^-1, thus enabling hyperspectral microscopy. Alongside CRS, it also features simultaneous detection of two-photon fluorescence and second harmonic generation for correlative studies. Our latest technology developments include high-content high-throughput label-free quantitative chemical imaging via Bessel beam illumination and sparse sampling. Our latest biological applications feature quantitative imaging of i) intra-cellular lipid droplets in live mouse oocytes and early embryos, ii) cancer organoids and their responses to drugs, iii) freshwater microalgae as potential food supplements for omega-3 fatty acids.

Original language	English
Title of host publication	2018 20th International Conference on Transparent Optical Networks, ICTON 2018
Publisher	IEEE Computer Society
Number of pages	4
Volume	2018-July
ISBN (Electronic)	978-1538666043
DOIs	https://doi.org/10.1109/ICTON.2018.8473706
Publication status	Published - 26 Sept 2018
Externally published	Yes
Event	20th International Conference on Transparent Optical Networks, ICTON 2018 - Bucharest, Romania Duration: 1 Jul 2018 → 5 Jul 2018

Conference

Conference	20th International Conference on Transparent Optical Networks, ICTON 2018
Country/Territory	Romania
City	Bucharest
Period	1/07/18 → 5/07/18

Keywords

bioimaging
coherent Raman scattering
multiphoton microscopy
vibrational microscopy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1109/ICTON.2018.8473706Licence: CC BY-NC-ND

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Pope, I., Masia, F., Bradley, J., Ewan, K., Karuna, A., Payne, L., Guschina, I., Harwood, J., Swann, K., Dale, T., Watson, P., Langbein, W., & Borri, P. (2018). Coherent Raman Scattering Microscopy: Technology Developments and Biological Applications. In 2018 20th International Conference on Transparent Optical Networks, ICTON 2018 (Vol. 2018-July). Article 8473706 IEEE Computer Society. https://doi.org/10.1109/ICTON.2018.8473706

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title = "Coherent Raman Scattering Microscopy: Technology Developments and Biological Applications",

abstract = "Optical microscopy is an indispensable tool that is driving progress in cell biology, and is still the only practical means of obtaining spatial and temporal resolution within living cells and tissues. Coherent Raman Scattering (CRS) microscopy has emerged in the last decade as a powerful multiphoton microscopy technique which overcomes the need for fluorescent labelling and yet retains biomolecular specificity and intrinsic 3D resolution. We have developed several home-built CRS microscopes featuring innovative excitation/detection schemes and quantitative image analysis. Our second-generation multimodal CRS instrument is based on a single 5fs Ti:Sa laser source with 350nm bandwidth capable of exciting a wide vibrational range from 1200 cm-1 to 3800 cm-1, thus enabling hyperspectral microscopy. Alongside CRS, it also features simultaneous detection of two-photon fluorescence and second harmonic generation for correlative studies. Our latest technology developments include high-content high-throughput label-free quantitative chemical imaging via Bessel beam illumination and sparse sampling. Our latest biological applications feature quantitative imaging of i) intra-cellular lipid droplets in live mouse oocytes and early embryos, ii) cancer organoids and their responses to drugs, iii) freshwater microalgae as potential food supplements for omega-3 fatty acids.",

keywords = "bioimaging, coherent Raman scattering, multiphoton microscopy, vibrational microscopy",

author = "I. Pope and F. Masia and J. Bradley and K. Ewan and A. Karuna and L. Payne and I. Guschina and J. Harwood and K. Swann and T. Dale and P. Watson and W. Langbein and P. Borri",

year = "2018",

month = sep,

day = "26",

doi = "10.1109/ICTON.2018.8473706",

language = "English",

volume = "2018-July",

booktitle = "2018 20th International Conference on Transparent Optical Networks, ICTON 2018",

publisher = "IEEE Computer Society",

note = "20th International Conference on Transparent Optical Networks, ICTON 2018 ; Conference date: 01-07-2018 Through 05-07-2018",

}

Pope, I, Masia, F, Bradley, J, Ewan, K, Karuna, A, Payne, L, Guschina, I, Harwood, J, Swann, K, Dale, T, Watson, P, Langbein, W & Borri, P 2018, Coherent Raman Scattering Microscopy: Technology Developments and Biological Applications. in 2018 20th International Conference on Transparent Optical Networks, ICTON 2018. vol. 2018-July, 8473706, IEEE Computer Society, 20th International Conference on Transparent Optical Networks, ICTON 2018, Bucharest, Romania, 1/07/18. https://doi.org/10.1109/ICTON.2018.8473706

Coherent Raman Scattering Microscopy: Technology Developments and Biological Applications. / Pope, I.; Masia, F.; Bradley, J. et al.
2018 20th International Conference on Transparent Optical Networks, ICTON 2018. Vol. 2018-July IEEE Computer Society, 2018. 8473706.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Coherent Raman Scattering Microscopy

T2 - 20th International Conference on Transparent Optical Networks, ICTON 2018

AU - Pope, I.

AU - Masia, F.

AU - Bradley, J.

AU - Ewan, K.

AU - Karuna, A.

AU - Payne, L.

AU - Guschina, I.

AU - Harwood, J.

AU - Swann, K.

AU - Dale, T.

AU - Watson, P.

AU - Langbein, W.

AU - Borri, P.

PY - 2018/9/26

Y1 - 2018/9/26

N2 - Optical microscopy is an indispensable tool that is driving progress in cell biology, and is still the only practical means of obtaining spatial and temporal resolution within living cells and tissues. Coherent Raman Scattering (CRS) microscopy has emerged in the last decade as a powerful multiphoton microscopy technique which overcomes the need for fluorescent labelling and yet retains biomolecular specificity and intrinsic 3D resolution. We have developed several home-built CRS microscopes featuring innovative excitation/detection schemes and quantitative image analysis. Our second-generation multimodal CRS instrument is based on a single 5fs Ti:Sa laser source with 350nm bandwidth capable of exciting a wide vibrational range from 1200 cm-1 to 3800 cm-1, thus enabling hyperspectral microscopy. Alongside CRS, it also features simultaneous detection of two-photon fluorescence and second harmonic generation for correlative studies. Our latest technology developments include high-content high-throughput label-free quantitative chemical imaging via Bessel beam illumination and sparse sampling. Our latest biological applications feature quantitative imaging of i) intra-cellular lipid droplets in live mouse oocytes and early embryos, ii) cancer organoids and their responses to drugs, iii) freshwater microalgae as potential food supplements for omega-3 fatty acids.

AB - Optical microscopy is an indispensable tool that is driving progress in cell biology, and is still the only practical means of obtaining spatial and temporal resolution within living cells and tissues. Coherent Raman Scattering (CRS) microscopy has emerged in the last decade as a powerful multiphoton microscopy technique which overcomes the need for fluorescent labelling and yet retains biomolecular specificity and intrinsic 3D resolution. We have developed several home-built CRS microscopes featuring innovative excitation/detection schemes and quantitative image analysis. Our second-generation multimodal CRS instrument is based on a single 5fs Ti:Sa laser source with 350nm bandwidth capable of exciting a wide vibrational range from 1200 cm-1 to 3800 cm-1, thus enabling hyperspectral microscopy. Alongside CRS, it also features simultaneous detection of two-photon fluorescence and second harmonic generation for correlative studies. Our latest technology developments include high-content high-throughput label-free quantitative chemical imaging via Bessel beam illumination and sparse sampling. Our latest biological applications feature quantitative imaging of i) intra-cellular lipid droplets in live mouse oocytes and early embryos, ii) cancer organoids and their responses to drugs, iii) freshwater microalgae as potential food supplements for omega-3 fatty acids.

KW - bioimaging

KW - coherent Raman scattering

KW - multiphoton microscopy

KW - vibrational microscopy

U2 - 10.1109/ICTON.2018.8473706

DO - 10.1109/ICTON.2018.8473706

M3 - Conference contribution

AN - SCOPUS:85055533757

VL - 2018-July

BT - 2018 20th International Conference on Transparent Optical Networks, ICTON 2018

PB - IEEE Computer Society

Y2 - 1 July 2018 through 5 July 2018

ER -

Coherent Raman Scattering Microscopy: Technology Developments and Biological Applications

Abstract

Conference

Keywords

UN SDGs

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