Chemical Structure of the Lipid A component of Pseudomonas sp. strain PAMC 28618 from Thawing Permafrost in Relation to Pathogenicity

Han-Gyu Park, Ganesan Sathiyanarayanan, Cheol-Hwan Hwang, Da-Hee Ann, Jung-Ho Kim, Geul Bang, Kyoung Soon Jang, Hee Wook Ryu, Yoo Kyung Lee*, Yung-Hun Yang*, Yun-Gon Kim

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Climate change causes permafrost thawing, and we are confronted with the unpredictable risk of newly discovered permafrost microbes that have disease-causing capabilities. Here, we first characterized the detailed chemical structure of the lipid A moiety from a Pseudomonas species that was isolated from thawing arctic permafrost using MALDI-based mass spectrometric approaches (i.e., MALDI-TOF MS and MALDI-QIT-TOF MSn). The MALDI multi-stage mass spectrometry (MS) analysis of lipid A extracted from the Pseudomonas sp. strain PAMC 28618 demonstrated that the hexaacyl lipid A ([M-H]- at m/z 1616.5) contains a glucosamine (GlcN) disaccharide backbone, two phosphates, four main acyl chains and two branched acyl chains. Moreover, the lipid A molecule-based structural activity relationship with other terrestrial Gram-negative bacteria indicated that strain PAMC 28618 has an identical lipid A structure with the mesophilic Pseudomonas cichorii which can cause rot disease in endive (Cichorium endivia) and that their bacterial toxicities were equivalent. Therefore, the overall lipid A validation process provides a general strategy for characterizing bacteria that have been isolated from arctic permafrost and analyzing their respective pathogenicities.
Original languageEnglish
Article number2168
JournalScientific Reports
Volume7
Issue number1
Early online date19 May 2017
DOIs
Publication statusPublished - 1 Dec 2017
Externally publishedYes

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