Artificial microRNAs (amiRNAs) engineering - on how microRNA-based silencing methods have affected current plant silencing research

Tatiana Tatarinova, Gaurav Sablok, Álvaro L Pérez-Quintero, Mehedi Hassan, Camilo López

Research output: Contribution to journalArticlepeer-review

Abstract

In recent years, endogenous microRNAs have been described as important regulators of gene expression in eukaryotes. Artificial microRNAs (amiRNAs) represent a recently developed miRNA-based strategy to silence endogenous genes. amiRNAs can be created by exchanging the miRNA/miRNA(*) sequence within a miRNA precursor with a sequence designed to match the target gene, this is possible as long as the secondary RNA structure of the precursor is kept intact. In this review, we summarize the basic methodologies to design amiRNAs and detail their applications in plants genetic functional studies as well as their potential for crops genetic improvement.
Original languageEnglish
Pages (from-to)315 - 319
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume406
Issue number3
DOIs
Publication statusPublished - 15 Feb 2011

Keywords

  • amirnas
  • gene knockouts
  • virus
  • model organism

Fingerprint

Dive into the research topics of 'Artificial microRNAs (amiRNAs) engineering - on how microRNA-based silencing methods have affected current plant silencing research'. Together they form a unique fingerprint.

Cite this