TY - JOUR
T1 - Addressing the challenge of optimum polyhydroxyalkanoate harvesting
T2 - Monitoring real time process kinetics and biopolymer accumulation using dielectric spectroscopy
AU - Kedia, Gopal
AU - Guwy, Alan
AU - Esteves, Sandra
AU - Dinsdale, Richard
AU - Passanha, Pearl
AU - Lee, Matthew
PY - 2013/4/1
Y1 - 2013/4/1
N2 - In this study, dielectric spectroscopy was utilised to evaluate and define the optimum harvesting time for polyhydroxyalkanoates (PHA) production. It is essential to harvest PHA at the optimum time during fermentation for maximum yield, otherwise cells start degrading. Two carbon sources (acetic and butyric acids) were used in laboratory based experiments and a number of samples were measured ex situ for PHA production. The real-time measured capacitance in addition of identifying the cells growth phase, it correlated very well with ex situ measured PHA produced within the cells. The probe has proven to be a useful tool to assess process kinetics, to monitor real-time cell growth, PHA produced and defining the optimum harvesting time.
AB - In this study, dielectric spectroscopy was utilised to evaluate and define the optimum harvesting time for polyhydroxyalkanoates (PHA) production. It is essential to harvest PHA at the optimum time during fermentation for maximum yield, otherwise cells start degrading. Two carbon sources (acetic and butyric acids) were used in laboratory based experiments and a number of samples were measured ex situ for PHA production. The real-time measured capacitance in addition of identifying the cells growth phase, it correlated very well with ex situ measured PHA produced within the cells. The probe has proven to be a useful tool to assess process kinetics, to monitor real-time cell growth, PHA produced and defining the optimum harvesting time.
KW - polyhydroxyalkanoates production
KW - volatile fatty acids
KW - biorefining
KW - harvest time
KW - dielectric spectroscopy
U2 - 10.1016/j.biortech.2013.01.136
DO - 10.1016/j.biortech.2013.01.136
M3 - Article
C2 - 23500571
VL - 134
SP - 143
EP - 150
JO - Bioresource Technology
JF - Bioresource Technology
SN - 0960-8524
ER -