A dual transacylation mechanism for polyketide synthase chain release in enacyloxin antibiotic biosynthesis

Joleen Masschelein, Paulina K. Sydor, Christian Hobson, Rhiannon Howe, Cerith Jones, Douglas M. Roberts, Zhong Ling Yap, Julian Parkhill, Eshwar Mahenthiralingam, Gregory L. Challis

Research output: Contribution to journalArticlepeer-review

100 Downloads (Pure)

Abstract

Polyketide synthases assemble diverse natural products with numerous important applications. The thioester intermediates in polyketide assembly are covalently tethered to acyl carrier protein domains of the synthase. Several mechanisms for polyketide chain release are known, contributing to natural product structural diversification. Here, we report a dual transacylation mechanism for chain release from the enacyloxin polyketide synthase, which assembles an antibiotic with promising activity against Acinetobacter baumannii. A non-elongating ketosynthase domain transfers the polyketide chain from the final acyl carrier protein domain of the synthase to a separate carrier protein, and a non-ribosomal peptide synthetase condensation domain condenses it with (1S,3R,4S)-3,4-dihydroxycyclohexane carboxylic acid. Molecular dissection of this process reveals that non-elongating ketosynthase domain-mediated transacylation circumvents the inability of the condensation domain to recognize the acyl carrier protein domain. Several 3,4-dihydroxycyclohexane carboxylic acid analogues can be employed for chain release, suggesting a promising strategy for producing enacyloxin analogues.
Original languageEnglish
Pages (from-to)906-912
JournalNature Chemistry
Volume11
DOIs
Publication statusPublished - 23 Sept 2019

Keywords

  • Antimicrobials
  • BIosynthesis
  • Multienzyme complexes
  • Natural products

Fingerprint

Dive into the research topics of 'A dual transacylation mechanism for polyketide synthase chain release in enacyloxin antibiotic biosynthesis'. Together they form a unique fingerprint.

Cite this