Triazole biotin: a tight-binding biotinidase-resistant conjugate

Anne I. Germeroth, Jill R. Hanna, Rehana Karim, Franziska Kundel, Jonathan Lowther, Peter G. N. Neate, Elizabeth A. Blackburn, Martin A. Wear, Dominic J. Campopiano, Alison N. Hulme

    Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

    6 Wedi eu Llwytho i Lawr (Pure)

    Crynodeb

    The natural amide bond found in all biotinylated proteins has been replaced with a triazole through CuAAC reaction of an alkynyl biotin derivative. The resultant triazole-linked adducts are shown to be highly resistant to the ubiquitous hydrolytic enzyme biotinidase and to bind avidin with dissociation constants in the low pM range. Application of this strategy to the production of a series of biotinidase-resistant biotin-Gd-DOTA contrast agents is demonstrated.

    Iaith wreiddiolSaesneg
    Tudalennau (o-i)7700-7704
    CyfnodolynOrganic and Biomolecular Chemistry
    Cyfrol11
    Rhif cyhoeddi44
    Dynodwyr Gwrthrych Digidol (DOIs)
    StatwsCyhoeddwyd - 1 Ion 2013

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