Targeting of Receptor Activator of Nuclear Kappa B (RANK) in PC-3 Cells Increases Cell Proliferation and Matrix Adhesion In Vitro

Sioned Owen*, Andrew J. Sanders, Malcolm D. Mason, Wen G. Jiang

*Awdur cyfatebol y gwaith hwn

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

Crynodeb

Background: In Western societies, prostate cancer is the most frequently diagnosed cancer amongst men. Efforts to improve diagnosis and treatment remain a major focus and have been proven beneficial in the approach to localised disease. However, currently, metastatic disease management still remains palliative. Receptor activator of nuclear kappa B (RANK) has been extensively studied in bone biology and immunology, whilst several links have been made between RANK-positive breast cancer cells and disease progression. Its role in prostate cancer biology remains poorly understood, therefore the aim of this study was to explore the functional role of endogenously produced RANK in metastatic PC-3 prostate cancer cells in isolation and in response to hepatocyte growth factor (HGF). Materials and Methods: RANK expression was targeted using hammerhead ribozyme technology in PC-3 prostate cancer cells, and verified by polymerase chain reaction and western blot. A variety of in vitro functional assays were conducted, including cell proliferation and matrix adhesion in the presence of HGF. Results: Suppression of RANK expression was successfully targeted with anti-RANK hammerhead ribozyme transgenes, as verified by PCR and western blot. Reduced RANK expression resulted in significantly increased PC-3 cell proliferation (p

Iaith wreiddiolSaesneg
Tudalennau (o-i)1127-1134
Nifer y tudalennau8
CyfnodolynAnticancer research
Cyfrol36
Rhif cyhoeddi3
StatwsCyhoeddwyd - Maw 2016
Cyhoeddwyd yn allanolIe
DigwyddiadChina-United Kingdom Cancer (CUKC) Conference 2015 - National Museum, Cardiff, Y Deyrnas Unedig
Hyd: 17 Jul 201518 Jul 2015

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