TY - JOUR
T1 - On-line SERS detection of adenine in DNA based on the optofluidic in-fiber integrated GO/PDDA/Ag NPs
AU - Gao, Danheng
AU - Yang, Xinghua
AU - Teng, Pingping
AU - Kong, Depeng
AU - Liu, Zhihai
AU - Yang, Jun
AU - Luo, Meng
AU - Li, Zhanao
AU - Wen, Xingyue
AU - Yuan, Libo
AU - Li, Kang
AU - Bowkett, Mark
AU - Copner, Nigel
AU - Wang, Xiaozhang
PY - 2021/4/1
Y1 - 2021/4/1
N2 - In this paper, a novel type of in-fiber optofluidic Raman self-assembly graphene oxide (GO)/PDDA/Ag NPs SERS sensor for the adenine in DNA is proposed. The microstructured hollow fiber (MHF) has a channel and a suspended core in microscale, which allows the sample to flow into the fiber. Meanwhile, the GO/PDDA/Ag NPs SERS substrates are modified on the MHF core by chemical bond. When the sample is interacted with the evanescent field excited by the suspended core, the on-line SERS signals can be coupled back to the core and be detected. Here, we carry out on-line optofluidic detection on the adenine samples. The results show that the detection limit (LOD) of the samples of is about 1 × 10−14 M. The unwound DNA solution extracted from biological blood is injected into the MHF. Under the action of the hydrogen bond between PDDA with adenine, the adenine base in DNA is successfully detected and the interference of guanine, cytosine and thymine is eliminated. Significantly, this work provides an integrated self-assembled GO/PDDA/Ag NPs SERS adenine sensor in an optofluidic fiber. This rapid, convenient and sensitive Raman detection sensor of adenine in DNA is expected to have more potential applications in HIV, genetics and other fields.
AB - In this paper, a novel type of in-fiber optofluidic Raman self-assembly graphene oxide (GO)/PDDA/Ag NPs SERS sensor for the adenine in DNA is proposed. The microstructured hollow fiber (MHF) has a channel and a suspended core in microscale, which allows the sample to flow into the fiber. Meanwhile, the GO/PDDA/Ag NPs SERS substrates are modified on the MHF core by chemical bond. When the sample is interacted with the evanescent field excited by the suspended core, the on-line SERS signals can be coupled back to the core and be detected. Here, we carry out on-line optofluidic detection on the adenine samples. The results show that the detection limit (LOD) of the samples of is about 1 × 10−14 M. The unwound DNA solution extracted from biological blood is injected into the MHF. Under the action of the hydrogen bond between PDDA with adenine, the adenine base in DNA is successfully detected and the interference of guanine, cytosine and thymine is eliminated. Significantly, this work provides an integrated self-assembled GO/PDDA/Ag NPs SERS adenine sensor in an optofluidic fiber. This rapid, convenient and sensitive Raman detection sensor of adenine in DNA is expected to have more potential applications in HIV, genetics and other fields.
KW - in-fiber Raman sensor
KW - optofluidic microstructured optical fiber
KW - Adenine of DNA
U2 - 10.1016/j.snb.2021.129517
DO - 10.1016/j.snb.2021.129517
M3 - Article
SN - 0925-4005
VL - 332
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
M1 - 129517
ER -