This paper describes the design, micro-fabrication and testing of a novel Micro-Aerial Vehicle (MAV) that mimicking a real locust. Actual parameters of locust insect are used to create a micro-scale MAV that can replace the traditional types that mimicking dragonfly and birds. Based on the obtained results, the novel MAV crucial parameters are its weight and strength to take-off under normal locust performance parameters fashion. Computational Fluid Dynamics (CFD) simulations are carried out at angles of attack of 10°, 20° and 30° and flapping frequencies of 19 Hz, 24 Hz, 30 Hz, 35 Hz and 40 Hz to investigate the aerodynamic performance of this designed MAV and optimize its flapping frequency. The simulation results defined the frequency at which the MAV is capable of hovering and take-off. In addition, the simulation results showed that the MAV is able to utilize some lift enhancement mechanisms that are being actually used by insects. These results enhances the manufacturing process of future MAV’s, especially in the material selection and manufacturing method, and the transmission mechanism for flight.