Increasing polyhydroxyalkanoate (PHA) yields from Cupriavidus necator by using filtered digestate liquors

Pearl Passanha; Sandra R. Esteves; Gopal Kedia; Richard M. Dinsdale; Alan J. Guwy

doi:10.1016/j.biortech.2013.08.050

Increasing polyhydroxyalkanoate (PHA) yields from Cupriavidus necator by using filtered digestate liquors

Pearl Passanha^*, Sandra R. Esteves, Gopal Kedia, Richard M. Dinsdale, Alan J. Guwy

^*Awdur cyfatebol y gwaith hwn

Cyfadran Cyfrifiadureg, Peirianneg a Gwyddoniaeth

Allbwn ymchwil: Cyfraniad at gyfnodolyn › Erthygl › adolygiad gan gymheiriaid

38 Dyfyniadau (Scopus)

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The production of polyhydroxyalkanoates (PHAs) using digestate liquor as culture media is a novel application to extend the existing uses of digestates. In this study, two micro-filtered digestates (0.22. μm) were evaluated as a source of complex culture media for the production of PHA by Cupriavidus necator as compared to a conventional media. Culture media using a mixture of micro-filtered liquors from food waste and from wheat feed digesters showed a maximum PHA accumulation of 12.29. g/l PHA, with 90% cell dry weight and a yield of 0.48. g PHA/g VFA consumed, the highest reported to date for C. necator studies. From the analysis of the starting and residual media, it was concluded that ammonia, potassium, magnesium, sulfate and phosphate provided in the digestate liquors were vital for the initial growth of C. necator whereas copper, iron and nickel may have played a significant role in PHA accumulation.

Iaith wreiddiol	Saesneg
Tudalennau (o-i)	345-352
Nifer y tudalennau	8
Cyfnodolyn	Bioresource Technology
Cyfrol	147
Dyddiad ar-lein cynnar	14 Awst 2013
Dynodwyr Gwrthrych Digidol (DOIs)	https://doi.org/10.1016/j.biortech.2013.08.050
Statws	Cyhoeddwyd - Tach 2013

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10.1016/j.biortech.2013.08.050

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@article{ed2016cf9e754a1bbfdf2c67dfb9ded3,

title = "Increasing polyhydroxyalkanoate (PHA) yields from Cupriavidus necator by using filtered digestate liquors",

abstract = "The production of polyhydroxyalkanoates (PHAs) using digestate liquor as culture media is a novel application to extend the existing uses of digestates. In this study, two micro-filtered digestates (0.22. μm) were evaluated as a source of complex culture media for the production of PHA by Cupriavidus necator as compared to a conventional media. Culture media using a mixture of micro-filtered liquors from food waste and from wheat feed digesters showed a maximum PHA accumulation of 12.29. g/l PHA, with 90% cell dry weight and a yield of 0.48. g PHA/g VFA consumed, the highest reported to date for C. necator studies. From the analysis of the starting and residual media, it was concluded that ammonia, potassium, magnesium, sulfate and phosphate provided in the digestate liquors were vital for the initial growth of C. necator whereas copper, iron and nickel may have played a significant role in PHA accumulation.",

keywords = "Digestate liquors, Nutrients, Polyhydroxyalkanoate production, Trace metals, Volatile fatty acids",

author = "Pearl Passanha and Esteves, {Sandra R.} and Gopal Kedia and Dinsdale, {Richard M.} and Guwy, {Alan J.}",

note = "Funding Information: The authors acknowledge the financial support provided by the European Regional Development (Convergence) Fund for the CymruH2Wales project. Authors also acknowledge Ben Jobling Purser, Gary Shipley and Dr. Jamie Massanet-Nicolau (University of South Wales, UK) for providing the digestates and Dr. Myrto P. Zacharof and Dr. Bob Lovitt (Swansea University, UK) for the microfiltration process of the digestates and the University Of Wolverhampton (UK) for the ICP analysis of the digestates. ",

year = "2013",

month = nov,

doi = "10.1016/j.biortech.2013.08.050",

language = "English",

volume = "147",

pages = "345--352",

journal = "Bioresource Technology",

issn = "0960-8524",

publisher = "Elsevier",

}

TY - JOUR

T1 - Increasing polyhydroxyalkanoate (PHA) yields from Cupriavidus necator by using filtered digestate liquors

AU - Passanha, Pearl

AU - Esteves, Sandra R.

AU - Kedia, Gopal

AU - Dinsdale, Richard M.

AU - Guwy, Alan J.

N1 - Funding Information: The authors acknowledge the financial support provided by the European Regional Development (Convergence) Fund for the CymruH2Wales project. Authors also acknowledge Ben Jobling Purser, Gary Shipley and Dr. Jamie Massanet-Nicolau (University of South Wales, UK) for providing the digestates and Dr. Myrto P. Zacharof and Dr. Bob Lovitt (Swansea University, UK) for the microfiltration process of the digestates and the University Of Wolverhampton (UK) for the ICP analysis of the digestates.

PY - 2013/11

Y1 - 2013/11

N2 - The production of polyhydroxyalkanoates (PHAs) using digestate liquor as culture media is a novel application to extend the existing uses of digestates. In this study, two micro-filtered digestates (0.22. μm) were evaluated as a source of complex culture media for the production of PHA by Cupriavidus necator as compared to a conventional media. Culture media using a mixture of micro-filtered liquors from food waste and from wheat feed digesters showed a maximum PHA accumulation of 12.29. g/l PHA, with 90% cell dry weight and a yield of 0.48. g PHA/g VFA consumed, the highest reported to date for C. necator studies. From the analysis of the starting and residual media, it was concluded that ammonia, potassium, magnesium, sulfate and phosphate provided in the digestate liquors were vital for the initial growth of C. necator whereas copper, iron and nickel may have played a significant role in PHA accumulation.

AB - The production of polyhydroxyalkanoates (PHAs) using digestate liquor as culture media is a novel application to extend the existing uses of digestates. In this study, two micro-filtered digestates (0.22. μm) were evaluated as a source of complex culture media for the production of PHA by Cupriavidus necator as compared to a conventional media. Culture media using a mixture of micro-filtered liquors from food waste and from wheat feed digesters showed a maximum PHA accumulation of 12.29. g/l PHA, with 90% cell dry weight and a yield of 0.48. g PHA/g VFA consumed, the highest reported to date for C. necator studies. From the analysis of the starting and residual media, it was concluded that ammonia, potassium, magnesium, sulfate and phosphate provided in the digestate liquors were vital for the initial growth of C. necator whereas copper, iron and nickel may have played a significant role in PHA accumulation.

KW - Digestate liquors

KW - Nutrients

KW - Polyhydroxyalkanoate production

KW - Trace metals

KW - Volatile fatty acids

U2 - 10.1016/j.biortech.2013.08.050

DO - 10.1016/j.biortech.2013.08.050

M3 - Article

C2 - 23999264

AN - SCOPUS:84883370639

SN - 0960-8524

VL - 147

SP - 345

EP - 352

JO - Bioresource Technology

JF - Bioresource Technology

ER -

Increasing polyhydroxyalkanoate (PHA) yields from Cupriavidus necator by using filtered digestate liquors

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