TY - JOUR
T1 - Increasing polyhydroxyalkanoate (PHA) yields from Cupriavidus necator by using filtered digestate liquors
AU - Passanha, Pearl
AU - Esteves, Sandra R.
AU - Kedia, Gopal
AU - Dinsdale, Richard M.
AU - Guwy, Alan J.
N1 - Funding Information:
The authors acknowledge the financial support provided by the European Regional Development (Convergence) Fund for the CymruH2Wales project. Authors also acknowledge Ben Jobling Purser, Gary Shipley and Dr. Jamie Massanet-Nicolau (University of South Wales, UK) for providing the digestates and Dr. Myrto P. Zacharof and Dr. Bob Lovitt (Swansea University, UK) for the microfiltration process of the digestates and the University Of Wolverhampton (UK) for the ICP analysis of the digestates.
PY - 2013/11
Y1 - 2013/11
N2 - The production of polyhydroxyalkanoates (PHAs) using digestate liquor as culture media is a novel application to extend the existing uses of digestates. In this study, two micro-filtered digestates (0.22. μm) were evaluated as a source of complex culture media for the production of PHA by Cupriavidus necator as compared to a conventional media. Culture media using a mixture of micro-filtered liquors from food waste and from wheat feed digesters showed a maximum PHA accumulation of 12.29. g/l PHA, with 90% cell dry weight and a yield of 0.48. g PHA/g VFA consumed, the highest reported to date for C. necator studies. From the analysis of the starting and residual media, it was concluded that ammonia, potassium, magnesium, sulfate and phosphate provided in the digestate liquors were vital for the initial growth of C. necator whereas copper, iron and nickel may have played a significant role in PHA accumulation.
AB - The production of polyhydroxyalkanoates (PHAs) using digestate liquor as culture media is a novel application to extend the existing uses of digestates. In this study, two micro-filtered digestates (0.22. μm) were evaluated as a source of complex culture media for the production of PHA by Cupriavidus necator as compared to a conventional media. Culture media using a mixture of micro-filtered liquors from food waste and from wheat feed digesters showed a maximum PHA accumulation of 12.29. g/l PHA, with 90% cell dry weight and a yield of 0.48. g PHA/g VFA consumed, the highest reported to date for C. necator studies. From the analysis of the starting and residual media, it was concluded that ammonia, potassium, magnesium, sulfate and phosphate provided in the digestate liquors were vital for the initial growth of C. necator whereas copper, iron and nickel may have played a significant role in PHA accumulation.
KW - Digestate liquors
KW - Nutrients
KW - Polyhydroxyalkanoate production
KW - Trace metals
KW - Volatile fatty acids
U2 - 10.1016/j.biortech.2013.08.050
DO - 10.1016/j.biortech.2013.08.050
M3 - Article
C2 - 23999264
AN - SCOPUS:84883370639
VL - 147
SP - 345
EP - 352
JO - Bioresource Technology
JF - Bioresource Technology
SN - 0960-8524
ER -
