Gene amplification and qRT-PCR

Cerith Jones, Alain Filloux

    Allbwn ymchwil: Pennod mewn Llyfr/Adroddiad/Trafodion CynhadleddPennodadolygiad gan gymheiriaid

    Crynodeb

    This chapter includes methods for the use of the polymerase chain reaction (PCR) with Pseudomonas, and several specific tips for their successful application in this organism. The first part of the chapter includes methods for purifying genomic DNA from, and amplifying genes from, Pseudomonas, in addition to methods which describe how to prepare a cell lysate from Pseudomonas species for colony PCR reactions. The chapter continues with a switch in focus from DNA to RNA, describing methods for RNA isolation from Pseudomonas, cDNA generation, and finally q-RT-PCR to investigate relative changes in gene expression.

    Iaith wreiddiolSaesneg
    TeitlPseudomonas Methods and Protocols
    GolygyddionAlain Filloux, Juan-Luis Ramos
    Tudalennau457-468
    Nifer y tudalennau12
    Cyfrol1149
    ISBN (Electronig)978-1-4939-0473-0
    Dynodwyr Gwrthrych Digidol (DOIs)
    StatwsCyhoeddwyd - 2014

    Cyfres gyhoeddiadau

    EnwMethods in Molecular Biology
    CyhoeddwrSpringer
    Cyfrol1149
    ISSN (Argraffiad)1064-3745
    ISSN (Electronig)1940-6029

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