TY - JOUR
T1 - Effect of acetate concentration, temperature, pH and nutrient concentration on polyhydroxyalkanoates (PHA) production by glycogen accumulating organisms
AU - Catherine, Marie-Claire
AU - Guwy, Alan
AU - Massanet-Nicolau, Jaime
N1 - Funding Information:
The authors would like to acknowledge the Knowledge Economy Skills Scholarships scheme (KESS) for the financial support. The authors declare no conflict of interest.
Publisher Copyright:
© 2022 The Author(s)
PY - 2022/12/1
Y1 - 2022/12/1
N2 - Low PHA accumulation is one of the main limitations of the industrial production of polyhydroxyalkanoates (PHA) from mixed cultures. Different PHA yields and physical properties were obtained by varying culture conditions (acetate concentration, nitrogen and phosphate availability, temperature and pH). Glycogen accumulating organisms (GAOs), microorganisms able to create and store PHA, which use acetate as their sole carbon source, were obtained after selection from mixed culture from activated sludge. All PHA presented good adhesion properties and were soluble in halogenated and polar solvents. The maximum PHA storage rate (20 % of cell dry weight) was achieved with a concentration of 4 g/L acetate. The optimum temperature for PHA production was 23 °C (24 % of cell dry weight). Low nitrogen concentration increased the yield (22 %) while phosphate concentration and pH did not influence PHA production. PHA accumulation of almost 30 % was obtained with previously found optimum parameters.
AB - Low PHA accumulation is one of the main limitations of the industrial production of polyhydroxyalkanoates (PHA) from mixed cultures. Different PHA yields and physical properties were obtained by varying culture conditions (acetate concentration, nitrogen and phosphate availability, temperature and pH). Glycogen accumulating organisms (GAOs), microorganisms able to create and store PHA, which use acetate as their sole carbon source, were obtained after selection from mixed culture from activated sludge. All PHA presented good adhesion properties and were soluble in halogenated and polar solvents. The maximum PHA storage rate (20 % of cell dry weight) was achieved with a concentration of 4 g/L acetate. The optimum temperature for PHA production was 23 °C (24 % of cell dry weight). Low nitrogen concentration increased the yield (22 %) while phosphate concentration and pH did not influence PHA production. PHA accumulation of almost 30 % was obtained with previously found optimum parameters.
KW - Acetate
KW - GAO
KW - Mixed culture
KW - PHA
U2 - 10.1016/j.biteb.2022.101226
DO - 10.1016/j.biteb.2022.101226
M3 - Article
AN - SCOPUS:85138781612
VL - 20
JO - Bioresource Technology Reports
JF - Bioresource Technology Reports
SN - 2589-014X
M1 - 101226
ER -
