We predicted and analyzed miRNAs precursors and targets using transcriptome of sweet wormwood (Artemisia annua L.), which is a source of an anti-malarial agent artemisinin. Among predicted miRNA targets, 29 (57%) correspond to unknown proteins, 5 (10%) to SQUAMOSA promoter-binding protein, 3 (6%) to Transport Inhibitor Response 1 protein; all other categories comprise the remaining 27% of the sequences. These targets are involved in response to copper deficiency, involved in copper homeostasis, mediating Aux/IAA proteins proteasomal degradation and auxin-regulated transcription. Repression of auxin signaling restricts growth of bacteria P. syringae, suggesting importance of miRNA-mediated suppression of auxin signaling in bacterial resistance.
|Statws||Cyhoeddwyd - 31 Rhag 2011|