Cell type and conduction velocity of rat primary sensory neurons with calcitonin gene-related peptide-like immunoreactivity

P. W. McCarthy*, S. N. Lawson

*Awdur cyfatebol y gwaith hwn

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

183 Dyfyniadau (Scopus)

Crynodeb

An immunocytochemical double labelling study of L4 dorsal root ganglia from rats aged seven to 10 weeks was made with an antibody to calcitonin gene-related peptide and with RT97, an anti-neurofilament antibody which specifically labels the light neuron population. Peptide immunoreactivity was found in an average of 46.5% of all neurons. Sixty-two per cent of the small dark (RT97negative) and 30% of the light (RT97-positive) neuron populations contained the peptide. About one-third (32%) of the cells with peptide immunoreactivity were light cells and about two-thirds (68%) were small dark cells. Intracellular electrophysiological recordings were made in vitro from neurons in lumbar (L4, L5 and L6) dorsal root ganglia from six- to eight-week-old rats, followed by dye-injection and immunocytochemistry. This showed that conduction velocities of neurons with calcitonin gene-related peptide-like immunoreactivity ranged from 0.5 to 28.6 m/s. Seventy-three neurons were successfully processed. Of these, calcitonin gene-related peptide-like immunoreactivity was found in 46% of C-fibre neurons, 33% of Aδ-fibre neurons and in 17% of the Aα/β-fibre neurons. The peptide-like immunoreactivity was found in approximately 25% of all A-fibre neurons sampled.

Iaith wreiddiolSaesneg
Tudalennau (o-i)623-632
Nifer y tudalennau10
CyfnodolynNeuroscience
Cyfrol34
Rhif cyhoeddi3
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 1990

Ôl bys

Gweld gwybodaeth am bynciau ymchwil 'Cell type and conduction velocity of rat primary sensory neurons with calcitonin gene-related peptide-like immunoreactivity'. Gyda’i gilydd, maen nhw’n ffurfio ôl bys unigryw.

Dyfynnu hyn