Artificial microRNAs (amiRNAs) engineering - on how microRNA-based silencing methods have affected current plant silencing research

Tatiana Tatarinova, Gaurav Sablok, Álvaro L Pérez-Quintero, Mehedi Hassan, Camilo López

Allbwn ymchwil: Cyfraniad at gyfnodolynErthygladolygiad gan gymheiriaid

Crynodeb

In recent years, endogenous microRNAs have been described as important regulators of gene expression in eukaryotes. Artificial microRNAs (amiRNAs) represent a recently developed miRNA-based strategy to silence endogenous genes. amiRNAs can be created by exchanging the miRNA/miRNA(*) sequence within a miRNA precursor with a sequence designed to match the target gene, this is possible as long as the secondary RNA structure of the precursor is kept intact. In this review, we summarize the basic methodologies to design amiRNAs and detail their applications in plants genetic functional studies as well as their potential for crops genetic improvement.
Iaith wreiddiolSaesneg
Tudalennau (o-i)315 - 319
Nifer y tudalennau4
CyfnodolynBiochemical and Biophysical Research Communications
Cyfrol406
Rhif cyhoeddi3
Dynodwyr Gwrthrych Digidol (DOIs)
StatwsCyhoeddwyd - 15 Chwef 2011

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