Titration methodologies have been used for the many years for low cost routine monitoring of full scale anaerobic digestion plants. These methodologies have been correlated to indicate the carbonate alkalinity and the volatile fatty acids (VFA) content within digesters. Two commonly used two end-point titration methods were compared using a dataset of 154 samples from energy crop and animal slurry digestates and were shown to be inaccurate in the estimation of tVFA. Using this dataset correlated with HPLC VFA analysis, two empirical bivariate linear regression equations were derived, where the validation dataset showed an absolute tVFA mean error improvement from +/- 3386 and 3324 mg kg(-1) tVFA to +/- 410 and +/- 286 mg kg(-1) tVFA, respectively. The same equation was then applied to a food waste dataset where an absolute tVFA mean error was improved from +/- 3828 to +/- 576 mg kg(-1) tVFA. The newly derived titration equations can provide greater confidence in digester performance monitoring and are tools that can improve digester management. (C) 2014 Elsevier Ltd. All rights reserved.